Dr. Kevin Rhine
ALSA Postdoctoral Fellow
2022
Summary
Kevin received his B.A. in Biochemistry & Molecular Biology and M.A. in Biotechnology from Boston University in 2017. His undergraduate and Master's research was with Dr. Dean R. Tolan, and he discovered that the enzyme aldolase could simultaneously bind to cytoskeletal proteins and engage in glycolysis. He then earned his Ph.D in Biology with Dr. Sua Myong at Johns Hopkins University. Kevin's graduate research focused on the RNA binding protein FUS, and how FUS condensates were nucleated, regulated, and disassembled. A major part of his Ph.D work was on how ALS-linked mutations in FUS altered the physical properties of FUS condensates. Kevin joined Dr. Gene Yeo's lab in 2022, and his postdoc research focuses on how aging impacts the RNA and protein interactions of ALS proteins in motor neurons, especially in the context of aggregation and liquid-liquid phase separation. Outside of lab, Kevin enjoys playing tennis and golf, baking sweets, and taking care of his houseplants and cat.
Education
B.A. Biochemistry & Molecular Biology, Boston University
M.A. Biotechnology, Boston University, 2017
PhD Johns Hopkins, 2022
Publications
Niaki A. G.*, Sarkar J.*, Cai X., Rhine K., Vidaurre V., Guy B., Hurst M., Lee J. C., Koh H. R., Guo L., Fare C. M., Shorter J., Myong S. Loss of dynamic RNA interaction and aberrant phase separation induced by two distinct types of ALS/FTD-linked FUS mutations. Mol Cell, 2020 Jan 2;77(1):82-94.e4.
Rhine K., Vidaurre V., Myong S. RNA Droplets. Ann Rev Biophys, 2020 May 6;49:247-265.
Rhine K., Makurath M. A., Liu J., Lopez C., Catalan K. F., Skanchy S., Fare C. M., Shorter J., Ma Y., Ha T., Chemla Y. R., Myong S. ALS/FTLD-Linked Mutations in FUS Glycine Residues Cause Accelerated Gelation and Reduced Interactions with Wild-Type FUS. Mol Cell, 2020 Oct 27;S1097-2765(20)30721-8.
Rhine K., Skanchy S., Myong S. Single-molecule and ensemble methods to probe RNP condensate nucleation and properties. Methods, 2021 Feb 19:S1046-2023(21)00049-9.
Hui M. H.*, Rhine K.*, Tolan D. R. Actin Filament- and Wiskott-Aldrich Syndrome Protein-Binding Sites on Fructose-1,6-bisphosphate Aldolase are Functionally Distinct from the Active Site. Cytoskeleton (Hoboken) 2021 Apr;78(4):129-141.
Rhine K., Myong S. Single molecule probing of disordered RNA binding proteins. STAR Protocols, 2022 Jan 28;3(1):101131.
Rhine K.*, Dasovich M.*, Yoniles J.*, Badiee M., Skanchy S., Ganser L., Fare C. M., Shorter J., Leung A. K. L, Myong S. Poly(ADP-ribose) drives condensation of FUS via a transient interaction. Mol Cell, 2022 Mar 3;82(5):969-985.e11.
Rhine K., Al-Azzam N., Yu T., Yeo G. W. Aging RNA granule dynamics in neurodegeneration. Front Mol Biosci, 2022 Sep 16;9:991641.
Fare C. M., Rhine K., Lam A., Myong S., Shorter J. “Molecular determinants of Kapβ2 chaperone and disaggregation activity.” J Biol Chem, 2022 Dec 15;299(2):102806.
Rhine K., Odeh H. M., Shorter J., Myong S. Regulation of biomolecular condensates by poly(ADP-ribose). Chem Rev, 2023 Apr 28. Online ahead of print.
Street L. A.*, Rothamel K. L.*, Brannan K.*, Jin W., Bokor B., Dong K., Rhine K., Madrigal A., Al-Azzam N., Kim J. K., Ma Y., Abdou A., Wolin E., Doron-Mandel E., Ahdout J., Mujumdar M., Jovanovic M., Yeo G.W. "Large-scale map of RNA binding protein interactomes across the mRNA life-cycle." bioRxiv 2023 June 9. Preprint.
Mekonnen G.*, Rhine K.*, Paul T., Skanchy S., Ge Y., Djaja N., Myong S. The DEAD-box helicase DDX6 regulates RNA-mediated FUS condensation. In preparation.